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The role of 3-isopropylmalate methylation is unclear but may represent a metabolic branch at 3-isopropylmalate, where some of the material is taken in the pathway leading to leucine and some is taken in a pathway to the 3-isopropylmalate methyl ester, a molecule that provides a signal to switch from vegetative to invasive growth in response to amino acid starvation Gene Name: TMT1 Uniprot ID: P32643 Molecular weight: 34768.0 Reactions General function: Involved in adenosylmethionine decarboxylase activity Specific function: S-adenosylmethionine decarboxylase is essential for normal growth, sporulation, maintenance of ds-RNA virus, biosynthesis of spermine and spermidine Gene Name: SPE2 Uniprot ID: P21182 Molecular weight: 46232.0 Reactions General function: Involved in methyltransferase activity Specific function: Converts DDMQH2 into DMQH2 Gene Name: COQ5 Uniprot ID: P49017 Molecular weight: 34684.19922 Reactions S-adenosyl-L-methionine + 2-polyprenyl-6-methoxy-1,4-benzoquinol → S-adenosyl-L-homocysteine + 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol

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Statistical analysis Statistical analysis was performed by a non-parametric Kruskal-Wallis ANOVA test and, later, a Mann-Whitney U -test, to compare groups