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For the genetic screening, a yeast genomic DNA library (Lista et al., 2017) constructed by inserting ∼4 kb genomic DNA fragments (obtained by Sau3A partial digestion) at the unique Bam HI site in the replicative 2 μ multicopy pFL44L vector containing URA3 -marker, was transformed into a yeast strain overexpressing CYS4 (with pRS423 and pRS424 plasmids)
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