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The human GCLC expression plasmid was constructed using the Gateway Cloning system (Thermo Fisher Scientific) and the pCMV-SPORT6-GCLC vector containing a human GCLC cDNA clone (Horizon Discovery Catalog #: MHS6278-202759380, Clone ID: 6052162, NCBI GeneBank Accession: BC039894), the Gateway™ pDONR™221 Vector (Thermo Fisher Scientific), and the pLenti-CMV-Neo-DEST (Addgene Plasmid #17392) destination vector
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